Acquisition of Tomato spotted wilt virus by Adults of Two Thrips Species

Dynamics of Tomato spotted wilt virus Replication in the Alimentary Canal of Two Thrips Species.

ABSTRACT Transmission of Tomato spotted wilt virus (TSWV) is dependent on virus uptake in the midgut prior to virus movement to the salivary glands. Replication of TSWV in the alimentary canal of tobacco thrips (TT, Frankliniella fusca) and western flower thrips (WFT, F. occidentalis) was investigated by immunolocalization of the nonstructural protein (NSs) encoded by the small RNA of TSWV and ...

Tomato spotted wilt virus

Tomato spotted wilt virus.

Growers in Georgia first noted problems in their peppers and onions during the last week of March 1989. Thrips populations combined with further field symptoms and the verification of the disease triggered a massive insecticide spray program in Georgia. Monitor was first used in early May followed by combinations of Orthene, Cygon and Thiodan. Most fields had less than 1% infection prompting a ...

Tactics for management of thrips (Thysanoptera: Thripidae) and tomato spotted wilt virus in tomato.

Four studies were conducted in Georgia during spring 1999, 2000, 2001, and 2002 to evaluate various management tactics for reducing thrips and thrips-vectored tomato spotted wilt virus (TSWV) in tomato and their interactions relative to fruit yield. Populations of thrips vectors of TSWV, Frankliniella occidentalis (Pergande) and Frankliniella fusca (Hinds), were determined using flower and stic...

Viral genetic determinants for thrips transmission of Tomato spotted wilt virus.

Tomato spotted wilt virus (TSWV) is transmitted exclusively by thrips in nature. A reassortment-based viral genetic system was used to map transmissibility by thrips to the medium (M) RNA of TSWV. To locate determinants of thrips transmission in the M RNA, 30 single-lesion isolates (SLIs) were generated from a single TSWV isolate that was inefficiently transmitted by thrips. Three of the 30 SLI...